Antifungal Therapeutics and Resistance

Working Group of Fabio Gsaller

About

Our research focuses on the molecular investigation of antifungal drug mechanisms of action and to elucidate factors that contribute to antifungal resistance. We aim to acquire a more comprehensive understanding on the metabolic effects of antifungals as well as on the adaptive strategies employed by Aspergillus fumigatus to protect itself from antifungal compounds with the final goal to develop alternative strategies to treat fungal diseases.

- Transcriptional regulation based antifungal resistance
- Metabolic pathways targeted by antifungal drugs
- Novel tools facilitating genetic engineering
- Genetic manipulation of Aspergillus spp. - gene deletions, knock-ins, site-directed mutagenesis, conditional expression mutants
- Antifungal susceptibility testing - minimum-inhibitory-concentration determination, plate assay based resistance testing
- Gene expression analysis - reporter assays, qRT-PCR, Northern analysis
- Phenotypical analysis of mutant strains
- Microscopy - multicolor fluorescence microscopy, time-lapse
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